Mycorrhizae are most effective when applied at the early stages of plant development (e.g. on seeds, seedlings or transplants) as it takes at least 6 to 8 weeks for spore-based mycorrhizae to establish a mature and extensive mycorrhizal network. Once the initial connections are established, the mycorrhizal network made of filament-like extensions will capture and drive soil nutrients to the plant. This early application allows crops/plants to take full advantage of all the benefits mycorrhizae have to offer. Only one application of Myco Supreme is needed.